{"id":"c45b6855-baf0-4f58-a586-3c36fdc05361","slug":"gemini-initial-behavioral-results-after-full-severance-of-the-cervical-spinal-cord-in-mice","title":"GEMINI: Initial behavioral results after full severance of the cervical spinal cord in mice","authors":["C-Yoon Kim","Hanseul Oh","In-Kyu Hwang","Ki-Sung Hong"],"abstract":"Background: The GEMINI spinal cord fusion protocol has been developed to achieve a successful cephalosomatic anastomosis. Here, we report the preliminary data on the use of a fusogen [polyethylene glycol (PEG)] after full cervical cord transection in mice to facilitate the fusion of both ends of a sharply transected spinal cord. Methods: Cervical laminectomy and a complete, visually confirmed cervical cord (C 5) transection was performed on female albino mice (n = 16). In Group 1 (n = 8), a fusogen, (PEG) was used to bridge the gap between the cut ends of the spinal cord. Group 2 received the same spinal cord transection but was treated with saline. Outcome was assessed daily using a standard scale (modified 22-point Basso-Beattie-Bresnahan scale) and filmed on camera. Results: The PEG group (group 1) showed partial restoration of motor function after 4 weeks of observation; group 2 (placebo) did not recover any useful motor activity. Conclusion: In this preliminary experiment, PEG, but not saline, promoted partial motor recovery in mice submitted to full cervical transection.","thumbnailUrl":"https://sni-digital-videos.s3.amazonaws.com/articles/c45b6855-baf0-4f58-a586-3c36fdc05361/featured/hero-1782420886187.png","publishDate":"2016-09-19T00:00:00.000Z","doi":"10.4103/2152-7806.190474","categories":["Spine","Original Article"],"fullTextUrl":"https://surgicalneurologyint.com/api/articles/c45b6855-baf0-4f58-a586-3c36fdc05361/pdf"}